Inoculum Size Effect in Dimorphic Fungi: Extracellular Control of Yeast-Mycelium Dimorphism in Ceratocystis ulmi

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Inoculum size effect in dimorphic fungi: extracellular control of yeast-mycelium dimorphism in Ceratocystis ulmi.

We studied the inoculum size effect in Ceratocystis ulmi, the dimorphic fungus that causes Dutch elm disease. In a defined glucose-proline-salts medium, cells develop as budding yeasts when inoculated at > or = 10(6) spores per ml and as mycelia when inoculated at <10(6) spores per ml. The inoculum size effect was not influenced by inoculum spore type, age of the spores, temperature, pH, oxygen...

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Lipoxygenase inhibitors shift the yeast/mycelium dimorphism in Ceratocystis ulmi.

The yeast-mycelium dimorphism in Ceratocystis ulmi, the causative agent of Dutch elm disease, was switched by gossypol, nordihydroguaiaretic acid, and propylgallate. In each case the mycelial form was converted to the yeast form. These compounds are recognized lipoxygenase inhibitors. Inhibitors of cyclooxygenase and thromboxane synthetase did not cause mycelia to shift to the yeast form. We su...

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Ca(II)-calmodulin regulation of fungal dimorphism in Ceratocystis ulmi.

We have shown that Ca(II) ions, ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid, LaCl3, and six known calmodulin inhibitors shift the yeast-mycelium dimorphic potential of Ceratocystis ulmi. Our data are consistent with the conclusions that Ca(II)-calmodulin interaction is necessary for mycelial growth in C. ulmi and that the absence of this interaction leads to the yeast phase.

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2004

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.70.3.1356-1359.2004